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The 2 protein bands from 40 kDa to thirty kDa were absent right after 24 h incubation at 45 °C. For the rest of the bands, many concentrations of all three proteases retained protein bands comparable to their authentic molar masses (Determine 6a,b). The existence of bands at minimal molecular pounds in fish roe prior to hydrolysis was unpredicted. Within the report by Ghelichi et al. [8], insignificant peaks of a relatively very low molecular bodyweight range (3–fourteen kDa) were being observed in defatted carp roe homogenate, probably resulting from an autolytic procedure taking place in fish roe and/or maybe the defatting process leading to the formation of reduced-molecular-weight peptides.
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